Sida loo isticmaalo tuubada cryopreservation si cilmiyeysan oo sax ah
Isticmaalka tuubada cryopreservation waa cilmi, ma aha trilogy fudud sida furitaanka haanta nitrogen dareere ah, gelinaya tube cryopreservation iyo xidhitaan taangiga nitrogen dareere ah.Isticmaalka sayniska iyo saxda ah ee tuubooyinka ilaalinta waxay ka fogaan kartaa luminta shaybaarada waxayna ilaalin kartaa badbaadada tijaabiyaasha.
Isticmaalka tuubada cryopreservation waa cilmi, ma aha trilogy fudud sida furitaanka haanta nitrogen dareere ah, gelinaya tube cryopreservation iyo xidhitaan taangiga nitrogen dareere ah.Isticmaalka sayniska iyo saxda ah ee tuubooyinka ilaalinta waxay ka fogaan kartaa luminta shaybaarada waxayna ilaalin kartaa badbaadada tijaabiyaasha.
Tuubbada qaboojinta: tallaabooyinka qaboojinta
Unugyada ku maydh xal PBS oo horay loo sii kululeeyay, nuug xalka, oo ku dabool unugyada xal ay ku jiraan trypsin iyo EDTA (lakabka dareeraha khafiifka ah ayaa ku filan, xoogga saarista trypsin iyo EDTA waxay u baahan yihiin in la go'aamiyo iyadoo loo eegayo xariiqda unugga).
Ku dheji unugyada 37 ℃ 3-5 daqiiqo.
Ka dib marka unugyadu ka go'aan xagga hoose, ka-soo-baxa waa la joojiyaa, dhexdhexaadka ka kooban serum ayaa lagu daraa, unugyaduna si tartiib ah ayaa loogu dhejiyaa pipette.
Centrifuge ka joojinta unugga (500 xg, 5 min) iyo dib u celinta iyada oo dhexdhexaad ah serum ka kooban.
Tirada unugyada
Centrifuge ka joojinta unugga (500 xg, 5 daqiiqo), ka saar dufanka sare, oo dib u celi unugyada leh dhexdhexaad ka kooban serum mug habboon.
Isku qas unugyada iyo xalka cryopreservation (60% dhexdhexaad ah, 20% serum bovine uurjiifka, 20% DMSO) ee 1: 1 saamiga mugga, ka dibna u wareeji Cryo STM tuubada cryopreservation.Cufnaanta unugyada barafaysan waa 1-5 × 106 xabo/ml.
Cryo ka kooban unugyo STM tuubbada cryopreservation waxaa lagula talinayaa in ay ku qaboojiso heerka - 1 K/min, iyo tuubada cryopreservation waxaa la gelin karaa weel ay ku jiraan isopropanol at - 70 ℃.Haddii tuubada cryopreservation Cryo STM ay kaydiso muunado kale, kuwaas oo si toos ah loo dhigi karo -20 ℃, - 70 ℃ ama wajiga gaaska ee nitrogen dareere ah.Si loo hubiyo in muunada la qaboojiyey si isku mid ah, 4 mL iyo 5 mL Cryo Tuubbada sTM cryopreservation waxay u baahan tahay in la geliyo qaboojiyaha at 20 ℃ habeenkii, ka dibna loo wareejiyo wajiga gaaska - 70 ℃ ama nitrogen dareere ah.
Kadibna ku wareeji tuubada cryopreservation Cryo.sTM haanta nitrogen ee dareeraha ah.Si looga fogaado wasakhowga (sida mycoplasma) iyo tixgelinta badbaadada, fadlan dhig Cryo.sTM tuubada cryopreservation ee wajiga gaaska ee nitrogen dareeraha ah, ma aha wajiga dareeraha.
Sidee loo isticmaalaa tuubada cryopreservation si cilmiyeysan oo sax ah?Shirkaddayadu waxay ka kooban tahay xirfadlayaal leh asal warshadeed iyo waayo-aragnimo suuq qani ah si ay u bixiyaan alaabada cilmi-baarista sayniska nolosha iyo adeegyada cilmi-baarayaasha sayniska.Ma buuxin karto oo kaliya shuruudaha gaarka ah ee macaamiisha R & D ee noocyada alaabta iyo baakadaha, laakiin sidoo kale waxay dabooli karaan baahiyaha guud ee shirkadaha wax soo saarka ee dhammaan heerarka kala duwan ee yar yar, dhexdhexaad ah ilaa wax soo saarka ballaaran.
Waqtiga boostada: Nov-25-2022