Tusaalaha ururinta, kaydinta iyo shuruudaha gaadiidka ee tijaabooyinka caadiga ah

Tusaalaha ururinta, kaydinta iyo shuruudaha gaadiidka ee tijaabooyinka caadiga ah

1. Ururinta iyo ilaalinta shaybaarrada cudurada cudurada:

☛Qaybta barafaysan: Ka saar unugyada ku habboon kuna kaydi nitrogen dareere ah;

☛Qaybta paraffin: Ka saar unugyada ku habboon kuna kaydi 4% paraformaldehyde;

☛ Sawirada unugyada: Sawirada unugyada waxaa lagu hagaajiyay 4% paraformaldehyde 30 daqiiqo, ka dibna waxaa lagu bedelay PBS waxaana la dhex geliyay PBS waxaana lagu kaydiyay 4°C.

2. Ururinta iyo ilaalinta shaybaarada bayoloji molecular:

☛Neef cusub: jar muunada oo ku kaydi qaboojiyaha dareeraha ah ee nitrogen ama -80°C;

☛ Muunada paraffin: Ku kaydi heerkulka qolka;

Muunada dhiigga oo dhan: Qaado qaddar ku habboon oo ah dhiigga oo dhan oo ku dar EDTA ama tuubada ururinta xinjirowga lidka ku ah heparin;

☛Samballada dareeraha jidhka:-xawaare sare-sare si loo ururiyo wasakhda;

Muunadaha unugyada: Unugyada waxaa lagu sagootiyey TRIzol waxaana lagu kaydiyaa nitrogen dareere ah ama qaboojiyaha -80°C.

3. Ururinta iyo kaydinta muunadaha tijaabada borotiinka:

☛Neef cusub: jar muunada oo ku kaydi qaboojiyaha dareeraha ah ee nitrogen ama -80°C;

Muunada dhiigga oo dhan: Qaado qaddar ku habboon oo ah dhiigga oo dhan oo ku dar EDTA ama tuubada ururinta xinjirowga lidka ku ah heparin;

Muunadaha unugyada: Unugyada si buuxda ayaa loogu shubay dareeraha lysis unugga ka dibna waxaa lagu kaydiyaa nitrogen dareere ah ama qaboojiyaha -80°C.

4. Ururinta iyo kaydinta ELISA, radioimmunoassay, iyo shaybaarada tijaabada biochemical:

☛Serum (plasma) muunada: Qaado dhiiga dhan oo ku dar tuubada procoagulation (tubo anticoagulation), sentrifuge at 2500 rpm ilaa 20 daqiiqo, ka soo ururi supernatant, oo ku kaydi nitrogen dareere ah ama qaboojiyaha -80°C;

☛Sambalka kaadida: ka dhig saamiga 2500 rpm ilaa 20 daqiiqo, kuna kaydi qaboojiyaha dareeraha ah ee nitrogen ama -80°C;tixraac habkan loogu talagalay dareeraha thoracic iyo ascites, dareeraha cerebrospinal, iyo dareeraha lavage alveolar;

☛Samballada unugga: Marka la ogaado qaybaha sirta ah, ku samee saamiga 2500 rpm ilaa 20 daqiiqo kuna kaydi qaboojiyaha dareeraha ah ee nitrogen ama -80°C;marka la ogaado qaybaha unugyada gudaha, Ku qas ka joojinta unugga PBS oo qabooji oo dhalaali si isdaba joog ah si aad u burburiso unugyada una sii daayaan qaybaha gudaha.Centrifuge at 2500 rpm ilaa 20 daqiiqo ka dibna soo qaado heerka sare sida kor ku xusan;

☛Samballada unugyada: Ka dib marka la gooyo muunada, miisaan oo ku qabooji qaboojiyaha dareeraha ah ee nitrogen ama -80°C si hadhow loo isticmaalo.

5. Ururinta tijaabada dheef-shiid kiimikaadka:

Muunadda kaadida: Ku rid saamiga 2500 rpm ilaa 20 daqiiqo kuna kaydi qaboojiyaha dareeraha ah ee nitrogen ama -80°C;tixraac habkan loogu talagalay dheecaanka thoracic iyo ascites, dareeraha cerebrospinal, dheecaanka alveolar lavage, iwm.;

☛Ka dib marka la gooyo saamiga nudaha, miisaan oo ku qabooji nitrogen dareeraha ah ama qaboojiyaha -80 ° C si hadhow loo isticmaalo;