Tallaabada ugu horreysa ee tijaabada ELISA ee guuleysta—doorashada saxanka saxda ah ee ELISA

TheELISAsaxanku waa qalab lagama maarmaan u ah ELISA, baaritaanka difaaca jirka ee ku xiran enzyme-ka.Waxaa jira arrimo badan oo saameeya guusha tijaabooyinka ELISA.Doorashada qalabka saxda ah waa talaabada ugu horeysa.Doorashada microplate ku habboon waxay ka caawin doontaa tijaabada inay guuleysato.

Qalabka eeELISAsaxanku guud ahaan waa polystyrene (PS), polystyrene-na waxa uu leeyahay xasillooni kiimikaad oo liidata waxaana lagu milmi karaa noocyo kala duwan oo dareere organic ah (sida hydrocarbons caraf udgoon, hydrocarbons halogenated, iwm.), waxaana lagu daxaliyay asiidh xooggan iyo alkalis.Aan u adkaysan dufan oo si sahal ah midabkoodu isu beddelo ka dib marka lagu soo bandhigo iftiinka UV.

Waa maxay noocyadaELISAtaarikada ma jiraan?

✦Ku dooro midab

saxan hufan:ku habboon tirooyinka iyo tayada-waji- adag-immunoassays iyo tijaabooyin xidhidh;

Saxan cad:ku habboon is-luminescence iyo chemiluminescence;

Saxan madow:ku haboon immunoassays fluorescent iyo imtixaanada xidhidhka.

✦Ku dooro adiga oo xoogga isku xidhan

Saxan xidhid hooseeya:Waxay si aan qarsoodi ahayn ugu xidhaa borotiinada iyada oo loo marayo bonds hydrophobic.Waxay ku habboon tahay sida gudbiyaha adag ee borotiinnada macromolecular oo leh miisaan kelli ah> 20kD.Awoodeeda isku xidhka borotiinka waa 200 ~ 300ng IgG/cm2.

Saxan xidhid sare leh:Daawaynta dusha ka dib, awooddeeda isku xidhka borotiinka ayaa si weyn loo wanaajiyey, iyadoo gaadhaysa 300 ~ 400ng IgG/cm2, iyo miisaanka molecular ee borotiinka xidhxidhan ee ugu weyn waa> 10kD.

✦U kala sooc qaabka hoose

Hoosta fidsan:index refractive hooseeya, ku habboon in la ogaado akhristayaasha microplate;

Hoosta:Tusmada dib-u-celinta waa mid sarreeya, taas oo ku habboon in lagu daro, hamiyo, isku dhafka iyo hawlgallada kale.Waxaad si toos ah ugu arki kartaa isbeddelka midabka adigoo eegaya muuqaalka adigoon dul saarin akhristaha microplate si loo go'aamiyo in uu jiro falcelin difaac oo u dhigma.