Isticmaal Habka iyo Ka Hortagga Tuubbada Qaboojinta
Tijaabooyinka microbiological, hal qalab oo tijaabo ah ayaa inta badan la isticmaalaa, taas oo ah, tuubada cryopreservation.Si kastaba ha ahaatee, kakanaantahooda kala duwan awgeed, saamayntu aad bay u kala duwan tahay.Sababtan awgeed, hadda, inta badan shaybaarada Shiinaha waxay sameeyaan tuubooyinka ilaalinta bakteeriyada iyaga laftooda, taas oo aan kordhinaynin xoojinta shaqada, laakiin sidoo kale sababtoo ah xaddidaadaha xaaladaha kala duwan, saameynta ilaalinta bakteeriyada had iyo jeer maaha mid ku qanacsan.
Sidaa darteed, waxaa lagama maarmaan ah in la barto habka isticmaalka iyo taxadarka qaar ka mid ah tuubada cryopreservation, si ay u ciyaaraan door weyn.
1.Habka codsiga
1).Markaad isticmaalayso tuubada cryopreservation si aad u kaydiso shaybaarada, waxaa si adag loogu baahan yahay in la geliyo tuubada cryopreservation lakabka uumiga ee nitrogen dareeraha ah ama qaboojiyaha si loo kaydiyo.Haddii tuubada cryopreservation lagu kaydiyo nitrogen dareere ah, waxaa jirta suurtogalnimo gaar ah in nitrogen dareeraha ah uu dhex galo tuubada cryopreservation.Inta lagu jiro soo kabashada, gaasta nitrogen dareeraha ah waxay u horseedi doontaa isku dheelitir la'aanta cadaadiska gudaha iyo dibadda, taas oo aad ugu dhow inay keento tuubada cryopreservation si ay u dillaacdo, waxayna leedahay khataro nafley ah.
2).Ku shaqee tuubada cryopreservation si aad dib ugu soo noolaato, oo isticmaal qalabka ilaalinta badbaadada inta ay socoto hawsha.Waxaa lagu talinayaa in la xidho dharka shaybaadhka, galoofyada suufka ah oo lagu qalo kursi shaybaar oo badbaado leh.Haddii ay suurtagal tahay, fadlan xidho muraayadaha indhaha ama gaashaanka wejiga.Maadaama heerkulka gudaha ee xagaaga uu ka sarreyn doono kan jiilaalka, fadlan taxaddar.
3).Inta lagu jiro kaydinta unugyada cryopreserved, heerkulka qaboojinta ee tuubooyinka cryopreserved waa in la mid ah.Qabow aan sinnayn ayaa u horseedi doonta macaanka baraf, kaas oo joojin doona gudbinta heerkulka dareeraha ah ee labada dhinacba, sidaas awgeed waxay dhalinaysaa cadaadis sare oo khatar ah oo waxyeelo gaadhsiiya tuubada qaboojinta.
4).Qadarka shaybaarka la qaboojiyey waa in aanu ka badnayn mugga shaqada ee ugu badan ee loo baahan yahay tuubada barafaysan.
2. Arrimaha u baahan feejignaan
1).Deegaanka kaydinta tuubada qaboojinta
Tuubooyinka ilaalinta aan la isticmaalin waxaa lagu kaydin karaa heerkulka qolka ama 2-8 ℃ ilaa 12 bilood;Tuubada la tallaalay ee cryopreservation waxaa lagu kaydin karaa -20 ℃ waxayna saameyn wanaagsan ku yeelan kartaa ilaalinta cadaadiska 12 bilood gudahood;Tuubada la tallaalay ee cryopreservation waxa lagu kaydin karaa -80 ℃, culayskana si fiican ayaa loo ilaalin karaa 24 bilood gudahood.
2).Waqtiga kaydinta tuubada qaboojinta
Tuubooyinka ilaalinta aan la isticmaalin waxaa lagu kaydin karaa heerkulka qolka ama 2-8 ℃;Tuubada la tallaalay ee cryopreservation waa in lagu kaydiyaa -20 ℃ ama -80 ℃.
3).Tallaabooyinka hawlgalka ee tuubada qaboojinta
Qaado dhaqamo cusub oo ka yimid dhaqamada bakteeriyada saafiga ah si aad u diyaarisid joojinta bakteeriyada oo leh turbidity qiyaastii 3-4 saamiga McDonnell ee tallaalka iyo tuubada ilaalinta cadaadiska;Adkee tuubada ilaalinta oo gadaal iyo gadaal u celi 4-5 jeer si aad bakteeriyada uga dhigto emulsified, iyada oo aan wareegin;Tuubada kaydinta geli talaajad si aad u ilaaliso (-20 ℃ – 70 ℃
Waqtiga boostada: Nov-25-2022